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Título: | Chemical sterilization of culture medium for in vitro multiplication of Cochlospermum regium |
Título(s) alternativo(s): | Esterilização química do meio de cultura para a multiplicação in vitro de Cochlospermum regium |
Palavras-chave: | Autoclaving Cerrado biome Active chlorine In vitro cultivation Autoclavagem Bioma cerrado Cloro ativo Cultivo in vitro |
Data do documento: | 2018 |
Editor: | Universidade Federal de Santa Maria |
Citação: | GAVILAN, N. H. et al. Chemical sterilization of culture medium for in vitro multiplication of Cochlospermum regium. Ciência Rural, Santa Maria, v. 48, n. 9, 2018. |
Resumo: | Cochlospermum regium roots are used in popular medicine and its extract has diverse phytochemical molecules some with antimicrobial activity, consequently exposing this specie to genetic erosion risks. Thus, the objective of this study was to develop an in vitro multiplication protocol using chemical sterilization of culture medium. Therefore, explants obtained from apical buds of C. regium seedlings were inoculated into with 0.05mg L-1 NAA and 1mg L-1 BAP sterilized by chemical agent sodium hypochlorite (NaOCl) at 0.001%, 0.003% and 0.005% of active chlorine (Cl). Autoclaved culture medium was used as control. Result showed that the contamination by bacterial at 91 days of cultivation was significantly (P<0.05) controlled by autoclaving, 0.001% and 0.005% Cl. Moreover, the callus induction in the culture medium with 0.001% and 0.005% Cl was, respectively, 30% and 20% major than autoclaving sterilization. There was not significant (P<0.05) in the percentage of shoot induction among the sterilization preparations methods, and 65% of the explants survived in the presence of culture medium with 0.005% Cl. Histological analyses indicated that the Cl did not have any deleterious effects on morphogenic events. These results indicated that the chemical sterilization using 0.001% - 0.005% Cl controlled the fungal and bacterial multiplication in the culture medium and no affected the C. regium explants development, becoming it an alternative to autoclaving method. |
URI: | http://repositorio.ufla.br/jspui/handle/1/33764 |
Aparece nas coleções: | DCF - Artigos publicados em periódicos |
Arquivos associados a este item:
Arquivo | Descrição | Tamanho | Formato | |
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ARTIGO_Chemical sterilization of culture medium for in vitro multiplication of Cochlospermum regium.pdf | 2,29 MB | Adobe PDF | Visualizar/Abrir |
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