Use este identificador para citar ou linkar para este item: http://repositorio.ufla.br/jspui/handle/1/33237
Título: Comparative oligo-FISH mapping: an efficient and powerful methodology to reveal karyotypic and chromosomal evolution
Palavras-chave: Chromosome identification
Karyotype
Oligo-FISH
Chromosome painting
Translocation
Data do documento: Fev-2018
Editor: Genetics Society of America (GSA)
Citação: BRAZ, G. T. et al. Comparative oligo-FISH mapping: an efficient and powerful methodology to reveal karyotypic and chromosomal evolution. Genetics, [S.l.], v. 208, n. 2, p. 513-523, Feb. 2018. DOI: 10.1534/genetics.117.300344.
Resumo: Developing the karyotype of a eukaryotic species relies on identification of individual chromosomes, which has been a major challenge for most nonmodel plant and animal species. We developed a novel chromosome identification system by selecting and labeling oligonucleotides (oligos) located in specific regions on every chromosome. We selected a set of 54,672 oligos (45 nt) based on single copy DNA sequences in the potato genome. These oligos generated 26 distinct FISH signals that can be used as a “bar code” or “banding pattern” to uniquely label each of the 12 chromosomes from both diploid and polyploid (4× and 6×) potato species. Remarkably, the same bar code can be used to identify the 12 homeologous chromosomes among distantly related Solanum species, including tomato and eggplant. Accurate karyotypes based on individually identified chromosomes were established in six Solanum species that have diverged for >15 MY. These six species have maintained a similar karyotype; however, modifications to the FISH signal bar code led to the discovery of two reciprocal chromosomal translocations in Solanum etuberosum and S. caripense. We also validated these translocations by oligo-based chromosome painting. We demonstrate that the oligo-based FISH techniques are powerful new tools for chromosome identification and karyotyping research, especially for nonmodel plant species.
URI: http://www.genetics.org/content/208/2/513
http://repositorio.ufla.br/jspui/handle/1/33237
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