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Campo DCValorIdioma
dc.creatorSilva, Viviam de Oliveira-
dc.creatorPereira, Luciano José-
dc.creatorMurata, Ramiro Mendonça-
dc.date.accessioned2018-11-23T12:46:56Z-
dc.date.available2018-11-23T12:46:56Z-
dc.date.issued2017-
dc.identifier.citationSILVA, V. de O.; PEREIRA, L. J.; MURATA, R. M. Oral microbe-host interactions: influence of β-glucans on gene expression of inflammatory cytokines and metabolome profile. BMC Microbiology, [S. l.], v. 17, n. 53, p. 1-9, 2017.pt_BR
dc.identifier.urihttps://bmcmicrobiol.biomedcentral.com/articles/10.1186/s12866-017-0946-1pt_BR
dc.identifier.urihttp://repositorio.ufla.br/jspui/handle/1/31918-
dc.description.abstractBackground: The aim of this study was to evaluate the effects of β-glucan on the expression of inflammatory mediators and metabolomic profile of oral cells [keratinocytes (OBA-9) and fibroblasts (HGF-1) in a dual-chamber model] infected by Aggregatibacter actinomycetemcomitans. The periodontopathogen was applied and allowed to cross the top layer of cells (OBA-9) to reach the bottom layer of cells (HGF-1) and induce the synthesis of immune factors and cytokines in the host cells. β-glucan (10 μg/mL or 20 μg/mL) were added, and the transcriptional factors and metabolites produced were quantified in the remaining cell layers and supernatant. Results: The relative expression of interleukin (IL)-1-α and IL-18 genes in HGF-1 decreased with 10 μg/mL or 20 μg/mL of β-glucan, where as the expression of PTGS-2 decreased only with 10 μg/mL. The expression of IL-1-α increased with 20 μg/mL and that of IL-18 increased with 10 μg/mL in OBA-9; the expression of BCL 2, EP 300, and PTGS-2 decreased with the higher dose of β-glucan. The production of the metabolite 4-aminobutyric acid presented lower concentrations under 20 μg/mL, whereas the concentrations of 2-deoxytetronic acid NIST and oxalic acid decreased at both concentrations used. Acetophenone, benzoic acid, and pinitol presented reduced concentrations only when treated with 10 μg/mL of β-glucan. Conclusions: Treatment with β-glucans positively modulated the immune response and production of metabolites.pt_BR
dc.languageen_USpt_BR
dc.publisherSpringer Naturept_BR
dc.rightsrestrictAccesspt_BR
dc.sourceBMC Microbiologypt_BR
dc.subjectAggregatibacter actinomycetemcomitanspt_BR
dc.subjectPeriodontal diseasept_BR
dc.subjectHost responsept_BR
dc.subjectKeratinocytept_BR
dc.subjectFibroblastpt_BR
dc.subjectImmunomodulationpt_BR
dc.subjectDoença periodontalpt_BR
dc.subjectResposta do hospedeiropt_BR
dc.subjectQueratinócitopt_BR
dc.subjectFibroblastopt_BR
dc.titleOral microbe-host interactions: influence of β-glucans on gene expression of inflammatory cytokines and metabolome profilept_BR
dc.typeArtigopt_BR
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