Use este identificador para citar ou linkar para este item: http://repositorio.ufla.br/jspui/handle/1/15311
Título: Effects of Aspergillus spp. exogenous fibrolytic enzymes on in vitro fermentation of tropical forages
Palavras-chave: Fibrolytic enzymes
Gas production technique
In vitro digestibility
Tropical forages
Enzimas fibrolíticas
Técnica de produção de gás
Digestibilidade in vitro
Forragens tropicais
Data do documento: Set-2012
Editor: Wiley
Citação: FACHINNI, F. D. A. et al. Effects of Aspergillus spp. exogenous fibrolytic enzymes on in vitro fermentation of tropical forages. Journal of the Science of Food and Agriculture, London, v. 92, n. 12, p. 2569–2573, Sept. 2012.
Resumo: BACKGROUND: Cellulose and hemicellulose are quantitatively the most important structural carbohydrates present in ruminant diets. Rumen micro-organisms produce enzymes that catalyse their hydrolysis, but the complex network formed by structural carbohydrates and lignin reduces their digestibility and restricts efficient utilisation of feeds by ruminants. This study aimed to produce two enzymatic extracts, apply them in ruminant diets to determine the best levels for ruminal digestibility and evaluate their effects on in vitro digestibility. RESULTS: In experiment 1 a two-stage in vitro technique was used to examine the effects of different enzymatic levels of Aspergillus japonicus and Aspergillus terricola on tropical forages. Enzyme addition had minor effects on corn silage at the highest enzymatic level. In experiment 2 an in vitro gas production (GP) technique was applied to determine apparent in vitro organic matter digestibility and metabolisable energy. The addition of enzymes in GP showed interesting results. Good data were obtained using sugar cane and Tifton-85 hay supplemented with extracts of A. japonicus and A. terricola respectively. CONCLUSION: Overall, the study suggests that addition of crude extracts containing exogenous fibrolytic enzymes to ruminant diets enhances the effective utilisation of ruminant feedstuffs such as forages. c 2012 Society of Chemical Industry
URI: http://onlinelibrary.wiley.com/doi/10.1002/jsfa.5684/full
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