Use este identificador para citar ou linkar para este item: http://repositorio.ufla.br/jspui/handle/1/13250
Título: Adubação orgânica, micropropagação e validação analítica para doseamento de cardenolídeos totais em Digitalis purpúrea subs. heywoodii
Título(s) alternativo(s): Organic fertilization, micropropagation and analytical validation for determination of total cardenolides in Digitalis purpurea subs. heywoodii
Autores: Bertolucci, Suzan Kelly Vilela
Pinto, José Eduardo Brasil Pereira
Pinto, José Eduardo Brasil Pereira
Braga, Fernão Castro
Pádua, Rodrigo Maia de
Figueiredo, Ana Cristina da Silva
Palavras-chave: Plantas medicinais – Propagação in vitro
Radiação ultravioleta
Adubação orgânica
Agentes antivirais
Medicinal plants – Micropropagation
Ultraviolet radiation
Organic fertilizer
Antiviral agents
Digitalis purpúrea subs. heywoodii
Data do documento: 22-Jun-2017
Editor: Universidade Federal de Lavras
Citação: SILVA, G. M. da. Adubação orgânica, micropropagação e validação analítica para doseamento de cardenolídeos totais em Digitalis purpúrea subs. heywoodii. 2017. 105 p. Tese (Doutorado em Plantas Medicinais, Aromáticas e Condimentares)-Universidade Federal de Lavras, Lavras, 2017.
Resumo: Digitalis purpurea subsp. heywoodii (Plantaginaceae) is considered a medicinally important species due to the presence of glycoevatromonoside cardenolide, responsible for its antiviral potential for human herpes. Its field cultivation presents a few obstacles, demanding the use of organic fertilization and in vitro cultivation as alternative for this issue. Thus, the objective was to cultivate the D. purpurea subsp. heywoodii species in pots with different doses of organic fertilization, as well as in vitro under the influence of physical and chemical factors, in order to evaluate its effects over growth and content of total cardenolides. The method for quantifying total cardenolides by UV spectrophotometry was validated, with selection at 494 nm for not presenting significant absorption in reading solution without the addition of coloring reagent, remaining linear in the range from 0.025 to 0.125 mg/mL, R2=0.9994. Interday (DPR = 3.26%) and intra-day (DPR = 3.58%) precision were considered exact from low to intermediate concentrations in the linear interval (98.4% and 98.9%), and robust for the reaction time of 25 min, with small variations in the length of the reading wave. The organic fertilization evaluated was chicken manure. The doses of this manure that better provided number and gain of foliar biomass were of 6 and 9 kg m-2. The absence of fertilizer provided greater root biomass gains, higher R/PA relation and higher average contents of cardenolides. However, cardenolide productivity were superior with the dose of 6 kg m-2. Concerning the chemical factors, different concentrations and combinations of cytokines BAP and TDZ (mg L-1) were used, in a total of thirteen treatments. A higher number of sprouts occurred with the BAP + TDZ combinations of 0.5 + 0.5; 0.25 + 0.75; 1.0 + 0.5 mg L-1. The average accumulation of dry biomass and productivity of total cardenolides was favored with the combination of 0.375 + 0.125 mg L-1. The effect of physical factors, such as intensity, quality and light shock were also evaluated. Intensity and light shock with 139 μmol m-2 s-1 produced greater the aerial part, root and total biomass, as well as higher content and productivity of total cardenolides. Blue LEDs favored both biomass production of the aerial part and productivity of total cardenolides. Therefore, the method was linear, precise, exact, robust, selective and reproducible, with ease of execution. The application of 6 kg m-2 of chicken manure in in vitro cultivation; the combination of 0.375 + 0.125 mg L-1 of BAP + TDZ supplemented to a MS culture medium; intensity and light shock with 139 μmol m-2 s-1; and blue LED in in vitro cultivation provided good conditions for the cultivation of the species, with increments in the production of biomass and productivity of total cardenolides.
URI: http://repositorio.ufla.br/jspui/handle/1/13250
Aparece nas coleções:Plantas Medicinais, Aromáticas e Condimentares - Doutorado (Teses)



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